Light Sheet Microscopy Optimized for Depth Penetration to Study Embryogenesis
نویسندگان
چکیده
منابع مشابه
Light-sheet optimization for microscopy
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM)...
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To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varyin...
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Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging w...
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Total internal reflection fluorescence microscopy (TIRF), in both commercial and custom-built configurations, is widely used for high signal-noise ratio imaging. The imaging depth of traditional TIRF is sensitive to the incident angle of the laser, and normally limited to around 100 nm. In our paper, using a high refractive index material and the evanescent waves of various waveguide modes, we ...
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1726-Pos Board B618 Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy Thai V. Truong, Daniel B. Holland, Vikas Trivedi, Scott E. Fraser. California Institute of Technology, Pasadena, CA, USA. Two-photon light sheet microscopy combines nonlinear excitation with the novel sheet-illumination, orthogonal to the detection direction, to achieve ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.4182